Difference between revisions of "CSC334 Lab2"
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=Retrieving Protein sequences from ExPASy= | =Retrieving Protein sequences from ExPASy= | ||
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PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI | PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI | ||
FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ | FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ | ||
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| + | © D. Thiebaut 2008 | ||
Revision as of 08:15, 24 July 2008
Retrieving Protein sequences from ExPASy
ExPASy is a database of proteins created by a pioneer of bioinformatics: Amos Bairoch
Methodology
- Point your browser to www.expasy.org/sprot/ and enter DUT_ECOLI in the search bar and click on Go:
- The Accession Number of the protein found is P06968. Its name in the Swiss-Prot database is DUT_ECOLI, but it is also known as EC 3.6.1.23, or dUTPase, or dUTP pyrophospatase.
- Find the Sequence Information at the bottom of the page, and select P06968 in FASTA format for the display. You should get something like this:
>sp|P06968|DUT_ECOLI Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Escherichia coli (strain K12) GN=dut PE=1 SV=1 MKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAPGDTTLVPTGLAIHIAD PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ
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© D. Thiebaut 2008
