Difference between revisions of "CSC334 Lab2"

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<meta name="keywords" content="computer science, bioinformatics, DNA, CSC334, Lab" />
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<meta name="description" content="DNA Sequence Logo Lab" />
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<meta name="abstract" content="DNA Sequence Logo" />
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<meta name="author" content="thiebaut at cs.smith.edu" />
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[[Csc334_Labs | Back to CSC334 Lab Page]]
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=Retrieving Protein sequences from ExPASy=
 
=Retrieving Protein sequences from ExPASy=
  
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[[Image:CSC334_Lab2_1.png | thumb | 300 px | frame | Figure 1]]
 
[[Image:CSC334_Lab2_1.png | thumb | 300 px | frame | Figure 1]]
  
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* Find the Sequence Information at the bottom of the page, and select P06968 in FASTA format for the display.  You should get something like this:
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* The '''Accession Number''' of the protein found is '''P06968'''.  Its name in the Swiss-Prot database is DUT_ECOLI, but it is also known as EC 3.6.1.23, or dUTPase, or dUTP pyrophospatase.
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* Find the '''Sequence Information''' at the bottom of the page, and select '''P06968 in FASTA''' format for the display.  You should get something like this:
  
 
  >sp|P06968|DUT_ECOLI Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Escherichia coli (strain K12) GN=dut PE=1 SV=1
 
  >sp|P06968|DUT_ECOLI Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Escherichia coli (strain K12) GN=dut PE=1 SV=1
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  PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI
 
  PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI
 
  FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ
 
  FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ
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[[Csc334_Labs | Back to CSC334 Lab Page]] 
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&copy; D. Thiebaut 2008

Latest revision as of 18:11, 4 August 2008

<meta name="keywords" content="computer science, bioinformatics, DNA, CSC334, Lab" /> <meta name="description" content="DNA Sequence Logo Lab" /> <meta name="title" content="Bioinformatics Lab" /> <meta name="abstract" content="DNA Sequence Logo" /> <meta name="author" content="thiebaut at cs.smith.edu" />

Back to CSC334 Lab Page


Retrieving Protein sequences from ExPASy

ExPASy is a database of proteins created by a pioneer of bioinformatics: Amos Bairoch

Methodology


Figure 1











  • The Accession Number of the protein found is P06968. Its name in the Swiss-Prot database is DUT_ECOLI, but it is also known as EC 3.6.1.23, or dUTPase, or dUTP pyrophospatase.
  • Find the Sequence Information at the bottom of the page, and select P06968 in FASTA format for the display. You should get something like this:
>sp|P06968|DUT_ECOLI Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Escherichia coli (strain K12) GN=dut PE=1 SV=1
MKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAPGDTTLVPTGLAIHIAD
PSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMI
FVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ



Back to CSC334 Lab Page
© D. Thiebaut 2008